CORRELATION BETWEEN FERTILITY AND LEVELS OFPROTEIN IN SEMINAL PLASMA OF EGYPTIANBUFFALO BULLS

The Objective of this study was to measure the protein profiles of seminalplasma in Egyptian buffalo bulls semen and to examine their correlation withsemen characteristics of buffalo bulls in Mehalet Mousa . Semen of 20 buffalobulls ( dividedinto high and low fertile bull, 10 for each group,according to records of insemination) were collected twice weekly for 12weeks by artificial vagina . Semen characteristics (ejaculate volume, motility ,abnormalities, viability and sperm cell concentration were recorded . Seminalplasma was seperated by centrifugation , treated with cold ethanol and then
underwent SDS-polyacrylamide gel electro phoresis ( PAGE) . Fourteenprotein bands were Identified on the gel of these protein band . 25 KDa wassignificantly correlated with all semen parameters studied in high fertile group, while 63 KDa was significantly correlated with sperm motility and spermabnormalities in low fertile Group
 
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